Stabilisers

ALP stabiliser/diluent

Enzyme-ImmunoAssay (EIA) has become an applied technique for identification and determination of antigens (including small molecules, i.e. haptens) and antibodies. Among the enzymes currently used for the development of solid-phase immunoassays, both Alkline phosphatase (ALP) and horseradish peroxidase (HRP) has been mainly used in the preparation of enzyme conjugates. Generally speaking, ALP is less sensitive than HRP.

As a general rule, the protein molecule is most stable at low temperature and high protein concentrations. The trend of immunoassay-based in vitro diagnostic products is toward the format of ready-to-use liquid solution instead of concentrated liquid solution and air- or freeze-dried solid. The major advantages of liquid reagents are: -

It avoids the denaturation of proteins caused by drying;
It eliminates the chance for human error when dissolving or diluted the dried or concentrated reagents.

Until now, therefore, the disadvantage of the format was the inherent instability of the liquid ALP-conjugates. This problem has now been addressed!!! The Biopanda's ALP stabilizer can significantly extend the life of ALP conjugates, allowing consistent results over extended periods of time. So the stabilizer allows you:-

To provide pre-diluted, READY-TO-USE ALP conjugates;
To store ALP conjugates at stock solution concentrations.

ALP stabiliser/diluent (Product No.: ALP-SD-001)

A protein-based formulation in a proprietary buffer. The diluted AP-conjugates may be used in applications such as ELISA, ImmunoBlotting and IHC.