TMB Substractes

TMB solutions for ELISA Procedures

One-component TMB substrate for ELISA

This type of TMB solutions is supplied as a ready-to-use mono-component liquid, containing TMB and H₂O₂ in an acidic buffer. Liquid TMB produces a blue, soluble end product which can be read at 655 nm. The reaction can be stopped by using acid solution (e.g. 0.5 M H₂SO₄, 0.5 M HCl, 1 M H₃PO₄) for converting the blue radical to the yellow diimine and being read at 450 nm, which has been reported to increase the sensitivity of the assay.

• Post termination stability: no change of OD within one hour, and <10% drop of OD within 24 hours after the addition of stopping solution

Is a robust ELISA substrate for HRP-based EIA with a stability of 3 years at 4°C.

Offers higher optical densities than the TMB solution I. Stable for at least 3 years at 4°C.

Provides a higher signal than TMB solution I with a 3-year shelf-life at 4°C.