Stop Solutions for TMB

Our Stop Solutions are acid-based solutions for use with our TMB ELISA substrates. TMB reacts with peroxide in the presence of horseradish peroxidase to produce a soluble blue product. This reaction can be stopped by the addition of an equal amount of stop solution, resulting in a soluble yellow product that can be read at 450nm.

Supplied in liquid format. For general use to stop colour development in ELISA.
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Supplied in liquid format. This solution can prevent the formation of precipitate of black TMB end product in ELISA.
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This stopping solution is capable of retaining the OD reading for over 2 hours after stopping.